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plpc trf2 δb δm plasmid  (Addgene inc)


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    Addgene inc plpc trf2 δb δm plasmid
    Plpc Trf2 δb δm Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plpc+trf2+%CE%B4b/pAG416GAL-ccdB-Cerulean+(Plasmid+%2314387)/pmc10182325-35-0-6
    Average 91 stars, based on 1 article reviews
    plpc trf2 δb δm plasmid - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Construct:

    Article Title: TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends
    Article Snippet: The histone H2B-FRB expression plasmid was generated by cloning mouse H2B without a stop codon and FRB from Lyn-linker-FRB (Addgene plasmid 20147 ( )) into pMSCV vector. .. FKBP-TRF2 and FKBP-TRF2 ΔB plasmids were constructed by cloning FKBP from YFP-FKBP-Rac1CA (Addgene plasmid 20150 ( )) into vectors pLPC-TRF2 and pLPC-TRF2 ΔB. ..

    Cloning:

    Article Title: TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends
    Article Snippet: The histone H2B-FRB expression plasmid was generated by cloning mouse H2B without a stop codon and FRB from Lyn-linker-FRB (Addgene plasmid 20147 ( )) into pMSCV vector. .. FKBP-TRF2 and FKBP-TRF2 ΔB plasmids were constructed by cloning FKBP from YFP-FKBP-Rac1CA (Addgene plasmid 20150 ( )) into vectors pLPC-TRF2 and pLPC-TRF2 ΔB. ..

    Plasmid Preparation:

    Article Title: TRF2 Protein Interacts with Core Histones to Stabilize Chromosome Ends
    Article Snippet: The histone H2B-FRB expression plasmid was generated by cloning mouse H2B without a stop codon and FRB from Lyn-linker-FRB (Addgene plasmid 20147 ( )) into pMSCV vector. .. FKBP-TRF2 and FKBP-TRF2 ΔB plasmids were constructed by cloning FKBP from YFP-FKBP-Rac1CA (Addgene plasmid 20150 ( )) into vectors pLPC-TRF2 and pLPC-TRF2 ΔB. ..



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    plasmid plpc trf2 δb  (Addgene inc)
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    Pu-27 induces phosphorylation of H2AX (γH2AX). U937 cells and <t>ΔB-U937</t> cells treated with Pu-27 and were subjected to Western blot and FACS analysis. A, 3-day treatment of Pu-27 showed profound up-regulation of γH2AX expression in U937 cells but almost no changes in dB-U937 cells. Relative expressions of <t>TRF2</t> in U937 and ΔB-U937 cells were also shown. TRF2 expression is down-regulated in U937 cells and no changes in ΔB-U937 cells when treated with Pu-27 (consistent with RT-PCR data in Fig. 6). Bottom panel, relative expression presented in bar graphs. *, p < 0.05. B, by FACS analysis γH2AX has shown continued to be highly expressed from days 1–3, and again there was no significant change in ΔB-U937 cells. Bottom panels, relative expression presented in bar graphs. *, p < 0.05.
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    Pu-27 induces phosphorylation of H2AX (γH2AX). U937 cells and ΔB-U937 cells treated with Pu-27 and were subjected to Western blot and FACS analysis. A, 3-day treatment of Pu-27 showed profound up-regulation of γH2AX expression in U937 cells but almost no changes in dB-U937 cells. Relative expressions of TRF2 in U937 and ΔB-U937 cells were also shown. TRF2 expression is down-regulated in U937 cells and no changes in ΔB-U937 cells when treated with Pu-27 (consistent with RT-PCR data in Fig. 6). Bottom panel, relative expression presented in bar graphs. *, p < 0.05. B, by FACS analysis γH2AX has shown continued to be highly expressed from days 1–3, and again there was no significant change in ΔB-U937 cells. Bottom panels, relative expression presented in bar graphs. *, p < 0.05.

    Journal: The Journal of Biological Chemistry

    Article Title: c-Myc Quadruplex-forming Sequence Pu-27 Induces Extensive Damage in Both Telomeric and Nontelomeric Regions of DNA *

    doi: 10.1074/jbc.M113.505073

    Figure Lengend Snippet: Pu-27 induces phosphorylation of H2AX (γH2AX). U937 cells and ΔB-U937 cells treated with Pu-27 and were subjected to Western blot and FACS analysis. A, 3-day treatment of Pu-27 showed profound up-regulation of γH2AX expression in U937 cells but almost no changes in dB-U937 cells. Relative expressions of TRF2 in U937 and ΔB-U937 cells were also shown. TRF2 expression is down-regulated in U937 cells and no changes in ΔB-U937 cells when treated with Pu-27 (consistent with RT-PCR data in Fig. 6). Bottom panel, relative expression presented in bar graphs. *, p < 0.05. B, by FACS analysis γH2AX has shown continued to be highly expressed from days 1–3, and again there was no significant change in ΔB-U937 cells. Bottom panels, relative expression presented in bar graphs. *, p < 0.05.

    Article Snippet: Aliquots of 0.4 ml of cells were transfected with 2 μg of the plasmid pLPC TRF2 ΔB (Addgene), which contains the TRF2 gene in which the basic domain of amino acids 1–44 is deleted.

    Techniques: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction

    Pu-27 inhibits the molecules related to DNA damage repair machinery in U937. A, RT-PCR of U937 and ΔB-U937 cells treated with Pu-27 for 3 days. Pu-27 inhibits ATM, RAD17, RAD50, CHK14, and CHK2 but not H2AX, BRCA1, and telomerase reverse transcriptase in U937 cells. B, ΔB-U937 cells showed no changes of TRF2, TRF1, POT1, TIN2, RAD17, RAD50, and 53BP1; down-regulation of ATM; up-regulation of CHK1 and CHK2; and H2AX and BRCA1.

    Journal: The Journal of Biological Chemistry

    Article Title: c-Myc Quadruplex-forming Sequence Pu-27 Induces Extensive Damage in Both Telomeric and Nontelomeric Regions of DNA *

    doi: 10.1074/jbc.M113.505073

    Figure Lengend Snippet: Pu-27 inhibits the molecules related to DNA damage repair machinery in U937. A, RT-PCR of U937 and ΔB-U937 cells treated with Pu-27 for 3 days. Pu-27 inhibits ATM, RAD17, RAD50, CHK14, and CHK2 but not H2AX, BRCA1, and telomerase reverse transcriptase in U937 cells. B, ΔB-U937 cells showed no changes of TRF2, TRF1, POT1, TIN2, RAD17, RAD50, and 53BP1; down-regulation of ATM; up-regulation of CHK1 and CHK2; and H2AX and BRCA1.

    Article Snippet: Aliquots of 0.4 ml of cells were transfected with 2 μg of the plasmid pLPC TRF2 ΔB (Addgene), which contains the TRF2 gene in which the basic domain of amino acids 1–44 is deleted.

    Techniques: Reverse Transcription Polymerase Chain Reaction